Extensive surveys of various locations of Sindh comprising Thatta, Hyderabad, TandoAallahYar, Matiari and Nawabshah(Benazirabad)were conducted during year 2013-2014. A total of 26 banana orchards, showing typical symptoms of banana wilt as well healthy ones were surveyed. The roots and soil samples from the banana rhizosphere  were collected for the isolation and identification of suspected pathogen, Fusariumoxysporumcubense and the burrowing nematode (Rhadopholussimilis) that are causing the Panama wilt in banana plantations.