The soil samples were collected from underground vegetable fields of Punjab and Khyber Pukhtunkhwa provinces. The plant parasitic nematodes were extracted from soil samples by Cobb’s sieving and decanting method (Cobb, 1918) followed by modified Baermann technique (Baermann, 1917). After processing, the nematode genera and species were identified (Siddiqi, 2000). Tylenchorhynchus usmanensis is reported from the first time from Pakistan is briefly redescribed and illustrated.