Twenty local and imported soybean (Glycine max) genotypes were evaluated for susceptibility to root-gall nematode (RGN), Meloidogyne incognita race 2, under greenhouse conditions. Significant differences among the genotypes resistance degrees were achieved. Substantial variations existed among the soybean genetic resources with regard to the evaluated parameters. According to reduction percentage in reproduction factor (RPRF), the tested genotypes were ranked as resistant or susceptible to challenge nematode genus. The genotypes viz., G22, G111(100% RF) were found to be highly resistant (HR), genotypes ranked as resistant (R) viz., C97, H2, CV1, Clark, A7 (99.97, 99.96, 98.73, 98.29 and 97.06% RF, respectively), moderately resistant (MR) category was achieved by seven genotypes (G21, CV2, Carford, Katler, G35, C11 and AG in which RF values varied between 76.01-91.72%). Two genetic resources (Tano and A10) reacted as low resistant (LR) their RF were 65.22 and 72.20%, successively. Susceptible (S) reaction was possessed by two genotypes (G82 and A11), also highly susceptible (HS) response was registered by the rest two genotypes, L2b3 and Holiday which were the most suitable one that recorded the highest rates of reproduction 17.26, 23.23 and used as susceptible standard. In general, growth of the tested genotypes was negative to nematode infection. Results obtained in the present study could be used for the planning of crop rotation systems as well as the identification of resistance sources for breeding purposes.