The fields of banana (Musa sapientum L.) cultivations were surveyed for the prevalence of phytonematodes in Assiut governorate at middle of Upper Egypt. The encountered phytonematodes were identified during the survey belong to sixteen genera comprising sixteen genera belonging to nine families viz., Criconemella, Criconemoides, Ditylenchus, Helicotylenchus, Heterodera, Hoplolaimus, Longidorus, Meloidogyne, Paratylenchus, Pratylenchus, Radopholus, Rotylenchulus, Rotylenchus, Tylenchorhynchus, Tylenchus and Xiphinema. It was observed that there was variability in population density, frequency of occurrence and prominence value of some plant parasitic genera e.g., Meloidogyne in Abo-Teg and East Mangabad has the most prominence value (17.20 and 19.80) followed by Longidorus and Radopholus in Manfalot (17.20 and 15.36), respectively. The lowest prevalent genus was Heterodera which was absent in two surveyed localities viz., Abo-Teg and Assiut regions but present in Manfalot and East Mangabad with the prominence value 1.82 and 0.22 and population densities 43 and 11, respectively.