Three known species belonging to order Rhabditida were recovered from sewage and stagnant water with sediments from different location of Karachi, Sindh, Pakistan. In this study, two genera, Rhabditidoides and Butlerius and three species identified as Rhabditidoides stigmatus, Butlerius micans and Demaniella basili were recorded for the first time in Pakistan. The percentage occurrence of these genera was found as 50% each. These species were identified on the basis of morphological, as well as morphometric characters. In addition, brief description, measurement, line drawings, as well as photomicrographs for Pakistani population of these species are reported.