The pure compounds of mistletoe (Viscum album) were evaluated in order to determine their nematicidal activity against Meloidogyne incognita (the root-knot nematode). The seven pure bioactive compounds have been isolated and examined, from which VA 4 and VA7 had a potential effect against M. incognita by showing 90% mortality at 0.1 g/mL after 48 hours of exposure. The LC50 0.0149, 0.1575 μg/ml of (VA4 and VA7), respectively found to be significantly effective than compounds VA5 (LC50 = 0.1575 μg/ ml), VA 6 (LC50 =0.1575 μg/ml), VA2 (LC50 = 0.6269μg/ml and VA3 (LC50 = 0.25) VA1(LC50 = 0.3969μg/ ml). The findings indicated that the constituents of V. album have a natural potential to use as nematicides and introduction in eco-friendly management strategies.