Strawberry (Fragaria L.) cultivation is widely practiced in Kyrgyzstan, where agriculture plays a significant role in the economy. Plant parasitic nematodes (PPN) are serious pests of several plants worldwide including straw berry. So far, no record of any plant parasitic nematode (PPN) exists detailing the species found in strawberry growing areas of Kyrgyzstan. This research aimed to provide a faunistic and taxonomic record of PPNs from soil and plant samples collected from strawberry orchards in 10 different locations in Tokmok area of northern Kyrgyzstan during the summer of 2016. PPNs were identified according to morphological and morphometric characters. Eight species belonging to the orders Tylenchida and Aphelenchida (Nematoda) were identified: Bitylenchus dubius, Boleodorus thylactus, Filenchus filiformis, Helicotylenchus digonicus, Merlinius brevidens, Pratylenchus neglectus, Psilenchus hilarulus (Nematoda: Tylenchida) and Aphelenchoides fragariae (Nematoda: Aphelenchida). The most common species were H. digonicus and M. brevidens. This is a first record for all obtained species from strawberry growing areas in Kyrgyzstan. Moreover, 3 plant parasitic species (B. dubius, H. digonicus and A. fragariae) were revealed for the first time in the agricultural areas of Kyrgyzstan and among these, A. fragariae (strawberry foliar nematode) is a very important pest of strawberry.