Grapevine orchards were surveyed for the prevalence of plant parasitic nematodes in Maghagha, Samallot, Minia and Abokorkas Districts of Minia Governorate. Soil and root samples were collected during fruiting seasons of grapevine in 2012 and 2013. The nematodes encountered were identified as Meloidogyne spp., Helicotylenchus spp., Longidorus spp., Pratylenchus spp., Tylenchulus semipenetrans and Hoplolaimus spp. Community analysis of these plant parasitic nematodes showed variability in nematodes population densities, frequencies, biomass and importance values in different Districts during 2012 and 2013. Root-knot nematodes Meloidogyne spp. was the most prevalent genus in the entire plant parasitic nematode community having highest relative frequency (0.78 and 0.83) in both years, respectively. The lowest relative frequencies (0.31 and 0.40) were recorded for Hoplolaimus spp., and Longidorus spp., in 2012 and 2013, respectively, Helicotylenchus spp. (0.44) and Meloidogyne spp. (0.42) were found with highest relative density in 2012 and 2013, respectively while the lowest relative densities (0.01 and 0.02) was recorded for Hoplolaimus spp. in 2012 and 2013, respectively. The highest biomass (1.25) and the highest importance value (1.69) recorded for needle nematodes, Longidorus spp., during 2012 and 2013, respectively. The lowest relative biomass (0.056) in both years was observed for Meloidogyne spp. while Hoplolaimus spp. exhibited the least importance value (0.64 and 0.79) in 2012 and 2013, respectively.